AEROBIC EXERCISE REDUCES SEVERE ASTHMA PHENOTYPE

TOPIC: Allergy and Airway TYPE: Original Investigations PURPOSE: Severe asthma is characterized by higher rates of airway hyperresponsiveness concomitantly increased eosinophilic neutrophilic systemic inflammation in which bradykinin plays a central role. Aerobic exercise (AE) the main component pulmonary rehabilitation inhibiting mild moderate asthmatics. In addition, AE mouse models allergic proven to reduce inflammation, remodeling, hyperresponsiveness, controlling exacerbated Th2 immune response. However, whether could inhibit severe phenotype unknown METHODS: House dust mite (HDM; 200ug/mouse; 3x/week; 6 weeks) Dermatophagoides pteronisynuss was used. Eight C57Bl/6 male mice (Control; n=2x10), (Exercise; (Asthma; n=2x10) (Asthma+Exercise; were assessed after weeks protocol (2 initial intra-tracheal HDM without AE) followed 4 treadmill (1h/session, 5x/week, intensity) + (4 HDM) RESULTS: The results showed that reduced HDM-increased total leukocytes, eosinophils, neutrophils, lymphocytes, macrophages (p<0.01) bronchoalveolar lavage (BAL) monocytes whole blood. cytokines IL-1beta, IL-4, IL-5, IL-13, IL-17, IL-33, TNF-alpha both BAL plasma HDM+Ex group compared with group, while IL-10 increased. density peribronchial lymphocytes percentage collagen fibers, epithelial smooth muscle thickness group. levels lung homogenate expression at mRNA qRT-PCR B1 receptor (p<0.001) mechanics revealed improved resistance (RAW; p<0.001), tissue (HTIS; elastance (GTIS; p<0.001). once AE-induced release, we tested hypothesis bradykinin-induced cells (BEAS-2B), fibroblast (MRC5), freshly isolated human eosinophils neutrophils activation, measured IL-1beta release. demonstrated inhibited release BEAS-2B, MRC5, neutrophils. CONCLUSIONS: conclusion, can vivo vitro, further clinical trials confirm these findings asthmatic patients are guaranteed. CLINICAL IMPLICATIONS: possible involvement Bradikinin may be one keys understanding mechanisms aerobic physical action phenotype. DISCLOSURES: no disclosure on file for Francine Almeida; No relevant relationships Nilsa Regina Damaceno-Rodrigues, source=Web Response Fernanda Degobbi Tenorio Quirino Santos Lopes; RENILSON FERREIRA, Sara Hamaguchi, João Bosco Pesquero; 119 Maysa Alves Rodrigues Rangel, Anamei Silva-Reis, Rodolfo de Paula Vieira